Analyse result | advanced biochemistry

Question: Put the figures in the right order and analyse result of each figure around 400 words in each

Figure in picture 1. Membrane transport characteristics of Xenopus laevis oocytes transformed with clone#3992. mRNA encoding orf3992 was transcribed in vitro from plasmid pEP(clone#3992) and transformed into Xenopus laevis oocytes. (A) Sugar transport into oocytes in the presence and absence of 10 µM carbonyl cyanide m-chlorophenyl hydrazone (CCCP). Extracellular medium supplemented with 1 mM sugar was left with oocytes for 1 hour and then intracellular concentration of sugar was measured. (B) Measurement of beemolulose transport kinetics into oocytes in the presence of different sugars.


Figure In picture 2. Modifications to orf3992 within A. candicus. (A) Schematic showing CRISPR/Cas9 with guide RNA (gRNA) that targets orf3992 co-transformed into A. candicus. (B) Effect of CRISPR/Cas9 disruption of orf3992 gene on A. candicus growth characteristics. Sugars were added to minimal growth medium and A. candicus was grown for 12 hours in minimal medium plus sugar. (C) Schematic showing transformation of expression plasmid encoding orf3992 into A. candicus. (D) Effect of orf3992 overexpression on A. candicus growth in minimal medium plus sugars.


Figure in picture 3. Screening Austral candicus cDNA library. (A) cDNA library preparation diagram. mRNA was extracted from A. candicus, converted to cDNA via reverse-transcription, followed by cloning into an expression plasmid with promoter constitutively active within S. cerevisiae. Following transformation of plasmid library into S. cerevisiae, 109 clones were plated onto minimal medium with all amino acids added lacking any sugar but beemolulose. Resulting colonies were picked and grown in 96-well liquid format with the same medium as used for plating colonies. Clone #3992 was one of 20 colonies that showed growth in the 96-well plate, had the most robust growth, and was chosen for further study. (B) Growth of yeast strains on different sugars. Growth was in liquid minimal medium supplemented with sugar(s) indicated. In the right panel, equimolar combinations of beemolulose plus indicated sugar were supplemented into minimal growth medium.  

Figure in picture 4. Identification and cellular localisation of ORF3992 protein. (A) Sequencing of clone #3992 reveals an open reading frame (ORF3992) of 700 amino acids long with hydropathy plotting potentially showing transmembrane domains. (B) Adding FLAG-tag to C-terminus of ORF3992 followed by transformation into yeast strain W303. (C) SDS-PAGE analysis of cytoplasm, membrane, and nucleus fractions from (-) yeast cells alone and (+) yeast cells carrying pEP(clone #3992). (D) Western blot of cytoplasm, membrane, and nucleus fractions from (-) yeast cells alone and (+) yeast cells carrying pEP(clone #3992). Probed with a rabbit anti-FLAG tag primary antibody, followed by anti-rabbit secondary antibody, and visualised with enhanced chemiluminescence (ECL) ­reagent.

University Writings
Calculate your paper price
Pages (550 words)
Approximate price: -

Why Work with Us

Top Quality and Well-Researched Papers

We always make sure that writers follow all your instructions precisely. You can choose your academic level: high school, college/university or professional, and we will assign a writer who has a respective degree.

Professional and Experienced Academic Writers

We have a team of professional writers with experience in academic and business writing. Many are native speakers and able to perform any task for which you need help.

Free Unlimited Revisions

If you think we missed something, send your order for a free revision. You have 10 days to submit the order for review after you have received the final document. You can do this yourself after logging into your personal account or by contacting our support.

Prompt Delivery and 100% Money-Back-Guarantee

All papers are always delivered on time. In case we need more time to master your paper, we may contact you regarding the deadline extension. In case you cannot provide us with more time, a 100% refund is guaranteed.

Original & Confidential

We use several writing tools checks to ensure that all documents you receive are free from plagiarism. Our editors carefully review all quotations in the text. We also promise maximum confidentiality in all of our services.

24/7 Customer Support

Our support agents are available 24 hours a day 7 days a week and committed to providing you with the best customer experience. Get in touch whenever you need any assistance.

Try it now!

Calculate the price of your order

Total price:

How it works?

Follow these simple steps to get your paper done

Place your order

Fill in the order form and provide all details of your assignment.

Proceed with the payment

Choose the payment system that suits you most.

Receive the final file

Once your paper is ready, we will email it to you.

Our Services

No need to work on your paper at night. Sleep tight, we will cover your back. We offer all kinds of writing services.


Essay Writing Service

No matter what kind of academic paper you need and how urgent you need it, you are welcome to choose your academic level and the type of your paper at an affordable price. We take care of all your paper needs and give a 24/7 customer care support system.


Admission Essays & Business Writing Help

An admission essay is an essay or other written statement by a candidate, often a potential student enrolling in a college, university, or graduate school. You can be rest assurred that through our service we will write the best admission essay for you.


Editing Support

Our academic writers and editors make the necessary changes to your paper so that it is polished. We also format your document by correctly quoting the sources and creating reference lists in the formats APA, Harvard, MLA, Chicago / Turabian.


Revision Support

If you think your paper could be improved, you can request a review. In this case, your paper will be checked by the writer or assigned to an editor. You can use this option as many times as you see fit. This is free because we want you to be completely satisfied with the service offered.